Review



proteintech rabgef1  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Proteintech proteintech rabgef1
    SILAC quantitative proteomics reveals proteome-wide differences associated with diphthamide deficiency. (A) Silver stain of HEK293T WT and DPH4KO cell lysates. (B) Workflow of designed SILAC proteomics experiment to quantitatively compare the proteome between HEK293T WT and DPH4KO cells. (C) MS/MS spectrum of the −1 frameshifted peptide VPQSQAEADSGGLGAGGATPAGGR detected in the HEK293T DPH4KO sample. Sequence alignment of <t>RABGEF1</t> mRNA reading frames without or with the predicted −1 frameshifting event, showing the generation of VPQSQAEADSGGLGAGGATPAGGR peptide and a resulting nonsense mutation after −1 frameshifting. The slippery sequence is labeled in red. Theoretical trypsin-digested peptides from the original reading frame are highlighted in red boxes, and the detected −1 frameshifted peptide is highlighted in a blue box. (D) Venn diagram of the −1 frameshifting protein candidates from computational prediction and SILAC proteomics. 164 overlapping genes were identified by combining the two methods. (E) Volcano plot of SILAC quantitative proteomics. 164 identified targets are highlighted in purple, and RRM1 is highlighted in red.
    Proteintech Rabgef1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proteintech+rabgef1/pmc11503486-148-23-23?v=Proteintech
    Average 93 stars, based on 11 article reviews
    proteintech rabgef1 - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Loss of Diphthamide Increases DNA Replication Stress in Mammalian Cells by Modulating the Translation of RRM1"

    Article Title: Loss of Diphthamide Increases DNA Replication Stress in Mammalian Cells by Modulating the Translation of RRM1

    Journal: ACS Central Science

    doi: 10.1021/acscentsci.4c00967

    SILAC quantitative proteomics reveals proteome-wide differences associated with diphthamide deficiency. (A) Silver stain of HEK293T WT and DPH4KO cell lysates. (B) Workflow of designed SILAC proteomics experiment to quantitatively compare the proteome between HEK293T WT and DPH4KO cells. (C) MS/MS spectrum of the −1 frameshifted peptide VPQSQAEADSGGLGAGGATPAGGR detected in the HEK293T DPH4KO sample. Sequence alignment of RABGEF1 mRNA reading frames without or with the predicted −1 frameshifting event, showing the generation of VPQSQAEADSGGLGAGGATPAGGR peptide and a resulting nonsense mutation after −1 frameshifting. The slippery sequence is labeled in red. Theoretical trypsin-digested peptides from the original reading frame are highlighted in red boxes, and the detected −1 frameshifted peptide is highlighted in a blue box. (D) Venn diagram of the −1 frameshifting protein candidates from computational prediction and SILAC proteomics. 164 overlapping genes were identified by combining the two methods. (E) Volcano plot of SILAC quantitative proteomics. 164 identified targets are highlighted in purple, and RRM1 is highlighted in red.
    Figure Legend Snippet: SILAC quantitative proteomics reveals proteome-wide differences associated with diphthamide deficiency. (A) Silver stain of HEK293T WT and DPH4KO cell lysates. (B) Workflow of designed SILAC proteomics experiment to quantitatively compare the proteome between HEK293T WT and DPH4KO cells. (C) MS/MS spectrum of the −1 frameshifted peptide VPQSQAEADSGGLGAGGATPAGGR detected in the HEK293T DPH4KO sample. Sequence alignment of RABGEF1 mRNA reading frames without or with the predicted −1 frameshifting event, showing the generation of VPQSQAEADSGGLGAGGATPAGGR peptide and a resulting nonsense mutation after −1 frameshifting. The slippery sequence is labeled in red. Theoretical trypsin-digested peptides from the original reading frame are highlighted in red boxes, and the detected −1 frameshifted peptide is highlighted in a blue box. (D) Venn diagram of the −1 frameshifting protein candidates from computational prediction and SILAC proteomics. 164 overlapping genes were identified by combining the two methods. (E) Volcano plot of SILAC quantitative proteomics. 164 identified targets are highlighted in purple, and RRM1 is highlighted in red.

    Techniques Used: Multiplex sample analysis, Quantitative Proteomics, Silver Staining, Tandem Mass Spectroscopy, Sequencing, Mutagenesis, Labeling



    Similar Products

    proteintech rabgef1  (Proteintech)
    93
    Proteintech proteintech rabgef1
    SILAC quantitative proteomics reveals proteome-wide differences associated with diphthamide deficiency. (A) Silver stain of HEK293T WT and DPH4KO cell lysates. (B) Workflow of designed SILAC proteomics experiment to quantitatively compare the proteome between HEK293T WT and DPH4KO cells. (C) MS/MS spectrum of the −1 frameshifted peptide VPQSQAEADSGGLGAGGATPAGGR detected in the HEK293T DPH4KO sample. Sequence alignment of <t>RABGEF1</t> mRNA reading frames without or with the predicted −1 frameshifting event, showing the generation of VPQSQAEADSGGLGAGGATPAGGR peptide and a resulting nonsense mutation after −1 frameshifting. The slippery sequence is labeled in red. Theoretical trypsin-digested peptides from the original reading frame are highlighted in red boxes, and the detected −1 frameshifted peptide is highlighted in a blue box. (D) Venn diagram of the −1 frameshifting protein candidates from computational prediction and SILAC proteomics. 164 overlapping genes were identified by combining the two methods. (E) Volcano plot of SILAC quantitative proteomics. 164 identified targets are highlighted in purple, and RRM1 is highlighted in red.
    Proteintech Rabgef1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proteintech+rabgef1/pmc11503486-148-23-23?v=Proteintech
    Average 93 stars, based on 1 article reviews
    proteintech rabgef1 - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    SILAC quantitative proteomics reveals proteome-wide differences associated with diphthamide deficiency. (A) Silver stain of HEK293T WT and DPH4KO cell lysates. (B) Workflow of designed SILAC proteomics experiment to quantitatively compare the proteome between HEK293T WT and DPH4KO cells. (C) MS/MS spectrum of the −1 frameshifted peptide VPQSQAEADSGGLGAGGATPAGGR detected in the HEK293T DPH4KO sample. Sequence alignment of RABGEF1 mRNA reading frames without or with the predicted −1 frameshifting event, showing the generation of VPQSQAEADSGGLGAGGATPAGGR peptide and a resulting nonsense mutation after −1 frameshifting. The slippery sequence is labeled in red. Theoretical trypsin-digested peptides from the original reading frame are highlighted in red boxes, and the detected −1 frameshifted peptide is highlighted in a blue box. (D) Venn diagram of the −1 frameshifting protein candidates from computational prediction and SILAC proteomics. 164 overlapping genes were identified by combining the two methods. (E) Volcano plot of SILAC quantitative proteomics. 164 identified targets are highlighted in purple, and RRM1 is highlighted in red.

    Journal: ACS Central Science

    Article Title: Loss of Diphthamide Increases DNA Replication Stress in Mammalian Cells by Modulating the Translation of RRM1

    doi: 10.1021/acscentsci.4c00967

    Figure Lengend Snippet: SILAC quantitative proteomics reveals proteome-wide differences associated with diphthamide deficiency. (A) Silver stain of HEK293T WT and DPH4KO cell lysates. (B) Workflow of designed SILAC proteomics experiment to quantitatively compare the proteome between HEK293T WT and DPH4KO cells. (C) MS/MS spectrum of the −1 frameshifted peptide VPQSQAEADSGGLGAGGATPAGGR detected in the HEK293T DPH4KO sample. Sequence alignment of RABGEF1 mRNA reading frames without or with the predicted −1 frameshifting event, showing the generation of VPQSQAEADSGGLGAGGATPAGGR peptide and a resulting nonsense mutation after −1 frameshifting. The slippery sequence is labeled in red. Theoretical trypsin-digested peptides from the original reading frame are highlighted in red boxes, and the detected −1 frameshifted peptide is highlighted in a blue box. (D) Venn diagram of the −1 frameshifting protein candidates from computational prediction and SILAC proteomics. 164 overlapping genes were identified by combining the two methods. (E) Volcano plot of SILAC quantitative proteomics. 164 identified targets are highlighted in purple, and RRM1 is highlighted in red.

    Article Snippet: Primary antibodies from Cell Signaling Technology: RRM1(#8637), RPA32 (#35869), HA (#3724), eEF2 (#2332), γH2AX (#9718), GAPDH (#2118), α-tubulin (#3873); Abcam: p-RPA32 T21 (#ab61065); Proteintech: RABGEF1 (#12735-1-AP); Santa Cruz: β-actin (sc-47778); and Novus Biological: DPH4 (#NBP1–87969) were used for immunoblotting at 1:1000 dilution.

    Techniques: Multiplex sample analysis, Quantitative Proteomics, Silver Staining, Tandem Mass Spectroscopy, Sequencing, Mutagenesis, Labeling